512KD？这个蛋白太大了一点。。。我最多转过200多KD的，你试试看转过夜呢？
网上找了一篇文献，Plos One 2008
ApoB100-LDL Acts as a Metabolic Signal from Liver to Peripheral Fat Causing Inhibition of Lipolysis in Adipocytes
里面没有特别讲条铅薯件，但确实做了western,ApoB100和48都有，备激握估计转膜时间要很长很长，他们仿庆的条件如下：
Mouse plasma samples (2 μl) were fractionated by SDS-PAGE using 4% gels. The apoB proteins were detected with rabbit antiserum against mouse apoB (Biosite, Taby, Sweden). The binding of primary antibodies was assessed with horseradish peroxidase?labeled donkey anti-rabbit antibodies and ECL western blotting reagents (Amersham Biosciences, GE Healthcare Biosciences, Little Chalfont, Buckinghamshire, England).